The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is ...Use the Cell Signaling Technology Technical Documentation search tool to find Product Data Sheets, Certificates of Analysis (CoA), and Safety Data Sheets (SDS). Begin your search by entering the product number in the search field. Online publication of this documentation makes it easier for you to access it, and more environmentally friendly ...Neurotrophin signaling through these receptors regulates a number of physiological processes, such as cell survival, proliferation, neural development, and axon and dendrite growth and patterning (1). In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for ...Mar 14, 2023 · A complete, step-by-step walkthrough of the CUT&RUN Protocol included with the CUT&RUN Assay kit (#86652) from Cell Signaling Technology (CST). • Visit the ... Lane 1 is 10% input, lane 2 is USP18 (D4E7) Rabbit mAb, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900. Western blot analysis was perfomed using USP18 (D4E7) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. To Purchase # 4813. Cat.H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks (1). DNA damage, caused by ionizing radiation, UV-light, or radiomimetic agents, results in rapid phosphorylation of H2A.X at Ser139 by PI3K-like kinases, including ATM, ATR, and DNA-PK (2,3).Use the Cell Signaling Technology Technical Documentation search tool to find Product Data Sheets, Certificates of Analysis (CoA), and Safety Data Sheets (SDS). Begin your search by entering the product number in the search field. Online publication of this documentation makes it easier for you to access it, and more environmentally friendly ...We would like to show you a description here but the site won’t allow us.The innate immune system mainly consists of phagocytic cells, such as neutrophils, macrophages, natural killer cells, basophils, and others that target dangerous invading organisms. The cells of the adaptive immune system are T cells and B cells. Natural killer (NK) T cells have features of both innate and adaptive immune cells.p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene ...Videos & Webinars | Cell Signaling Technology. USA. Technical Support +1-877-678-8324. Contact Us. PhosphoSitePlus ®. Register Sign In. Quick Add.NKX2.5 plays an essential role in heart development and is among the earliest factors expressed in the cardiac lineage in developing embryos. Targeted disruption of the murine Nkx2.5 gene results in abnormal heart morphogenesis, severe growth retardation, and embryonic lethality around E9.5 (1,2). Cellular Landscapes. Explore these interactive renderings of cellular structures to understand how these structures and mechanisms function together to accomplish different cellular processes. Click each structure to discover related PTM sites, proteins, disease implications, and products. Visit our Posters page to request a printed copy of the ... Cyclin D is degraded through the ubiquitin proteasome pathway in the absence of mitogenic signaling. Ubiquitination of cyclin D1 is enhanced by phosphorylation at Thr286 by glycogen synthase kinase 3b (GSK-3b) (1). p27/Kip1, p57 Kip2 and p21 Waf1/Cip1 are members of the Cip/Kip family of cyclin-dependent kinase inhibitors.Monoclonal Antibody for studying H3 (Ser10) phosphate. Cited in 454 publications. Validated for WB, IF, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Histone H3 (Ser10) (6G3) Mouse Monoclonal Antibody (CST #9706) is …Confocal immunofluorescent images of HUVEC cells untreated (left) or stimulated with Vascular Endothelial Growth Factor (VEGF) #9943 (right) and labeled with Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb #2478 (top, green) and VEGF Receptor 2 (55B11) Rabbit mAb (bottom, green). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye). For reproducible data, Cell Signaling Technology (CST) offers rigorously validated and reliable products including antibodies, reagents, and kits for biological research. We have a substantial portfolio of products to interrogate mechanisms of cellular health and disease. Whether in the fields of cancer, immunology, infectious disease ... Poster: Signaling Pathways | Cell Signaling Technology. This poster provides a comprehensive overview of the major signaling pathways involved in cell growth, survival, differentiation, and stress responses. Download the PDF to explore the key molecules and interactions that regulate these pathways and their implications for human health and …Immunoprecipitation of ATF-6 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is ATF-6 (D4Z8V) Rabbit mAb. Western blot analysis was performed using ATF-6 (D4Z8V) Rabbit mAb. Western blot analysis of extracts from 293T cells, untreated (-) or treated with tunicamycin (1 mM ... Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase ... Background. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are ubiquitously expressed, controlling cell structure and motility (1). While all actin isoforms are highly homologous, cytoplasmic β- and γ ... Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows ...PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197 and 1217 is correlated with PLCγ2 activity (9,10). Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509. Smrcka, A.V. et al. (1991) Science 251, 804-7.The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is ...Specificity / Sensitivity. Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full-length caspase-3 or other cleaved caspases.Directions for Use: Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBST. Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well.PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197 and 1217 is correlated with PLCγ2 activity (9,10). Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509. Smrcka, A.V. et al. (1991) Science 251, 804-7.The Harper Lab studies mechanisms underlying cellular homeostasis and signaling, with a focus on the ubiquitin system and the autophagy-lysosome system. The interest in the …Contact Technical Support. Hours: 8:00 AM - 6:00 PM (EST) Phone: 877-678-8324. Email: [email protected], EQUITY, & INCLUSION. CST understands the importance of a safe and diverse workplace. Different perspectives and ideas drive collaboration and innovation. By providing an inclusive and stimulating work environment, we aim to establish human dignity and meaningful work as an essential part of our culture. Embrace the. culture.In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca 2+ /calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3).Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Monoclonal Antibody for studying Flag-Tag. Cited in 478 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, DYKDDDDK Tag (9A3) Mouse Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (CST #8146) is ready to ship.Background. TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4).Simple Western™ analysis of lysates (0.1 mg/mL) from Raji cells using Mcl-1 (D35A5) Rabbit mAb #5453. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 ... Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63411. Incubate membrane with Anti-mouse IgG, HRP-linked Antibody ( #7076 at 1:2000) and Anti-biotin, HRP-linked Antibody ( #7075 at 1:1000–1:3000) to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature. Wash three times for 5 min each with 15 ml of TBST. Proceed with detection (Section D). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Background. TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein and other cytokines including IL-1 (1,2). In vivo activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4).At Cell Signaling Technology (CST), we understand that there is no single assay that can determine the validity of an antibody.Confirming that an immunoreagent is sufficiently specific and sensitive depends on the application and protocol being used, the type and quality of sample being analyzed, and the inherent biophysical properties of the antibody itself.PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197 and 1217 is correlated with PLCγ2 activity (9,10). Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509. Smrcka, A.V. et al. (1991) Science 251, 804-7.Species Cross-Reactivity Key: Polyclonal Antibody for studying eNOS. Cited in 167 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, eNOS Antibody (CST #9572) is ready to ship.Dana Seehale joined Cell Signaling Technology in February 2017 as Director, Lab Informatics, responsible for multiple technologies supporting Product Development and Production, including PDP/PLM, Core LIMS, and Jira. Her responsibilities were later expanded to include the PIM/STEP system and the Oracle EBS/EPortal platforms. Dana is now ...If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on …The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. CUT&RUN was performed with 293 cells treated with Forskolin #3828 (30 μM) for 1h and Phospho-CREB (Ser133) (87G3) Rabbit mAb, using CUT&RUN Assay Kit #86652.Cell Signaling presents the principles and components that underlie all known signaling processes. It provides undergraduate and graduate students the conceptual tools needed …Contact Technical Support. Hours: 8:00 AM - 6:00 PM (EST) Phone: 877-678-8324. Email: [email protected] -Rabbit. All -All Species Expected. Polyclonal Antibody for studying H2AX (Ser139) phosphate. Cited in 1048 publications. Validated for WB, WB, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Histone H2A.X (Ser139) Antibody (CST #2577) is ready to ship.Keratin 19 (K19) is expressed in glandular epithelia, including the liver, gallbladder, and pancreas, as well as in adenocarcinomas of the breast, thyroid, and bile duct. Keratin 20 (K20) is expressed in gastrointestinal epithelium, urothelium, and Merkel cells in the skin, as well as in colorectal carcinomas and some urothelial carcinomas.NKX2.5 plays an essential role in heart development and is among the earliest factors expressed in the cardiac lineage in developing embryos. Targeted disruption of the murine Nkx2.5 gene results in abnormal heart morphogenesis, severe growth retardation, and embryonic lethality around E9.5 (1,2).Western blot analysis of extracts from various cell lines using GAPDH (14C10) Rabbit mAb. Simple Western™ analysis of lysates (0.01 mg/mL) from HeLa cells using GAPDH (14C10) Rabbit mAb #2118. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. Simple Western™ analysis of lysates (0.1 mg/mL) from Jurkat cells treated with Cytochrome C using Cleaved Caspase-3 (Asp175) Antibody #9661. The virtual lane view (left) shows two target bands (as indicated) at 1:10 and 1:50 dilutions of primary antibody. CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and …Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Apr 15, 2022 · Cell Signaling Technology (CST) emphasizes that it was founded by scientists, for scientists. This is at the core of the company’s culture and permeates all its decisions. Headed by neuroscientist Michael Comb, CST seeks to deeply understand the requirements of their customers—bench researchers who are in the weeds of complex research ... Immunoprecipitation of STING from HL-60 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or STING (D2P2F) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using STING (D2P2F) Rabbit mAb. Aliquoting of 10x buffer is recommended if many small experiments are to be performed. 2. Thaw 10x buffer at 24-30°C, mixing end-over-end. 3. Dilute 10X Cell Lysis Buffer to a 1X solution using ddH2O. This product supplies enough 10X material to make 150mls of whole cell extract. 4. Chill 1X buffer on ice and add PMSF just prior to use. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. Cathepsin B (D1C7Y) XP ® Rabbit mAb recognizes endogenous levels of total cathepsin B protein.Monoclonal Antibody for studying ACTA2 (alpha actin, smooth muscle). Cited in 560 publications. Validated for WB, WB, IP, IHC, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, α-Smooth Muscle Actin (D4K9N) XP® Rabbit Monoclonal Antibody (CST #19245) is ready to ship.1X Animal-Free Blocking Solution: to 4 mL of dH O add 1 ml of Animal-Free Blocking Solution (5X) ( #15019 ). Incubate sections in three washes of xylene for 5 minutes each. Incubate sections in two washes of 100% ethanol for 10 minutes each. Incubate sections in two washes of 95% ethanol for 10 minutes each.Directions for Use: Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBST. Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well.Wash sections in wash buffer for 5 min. Block each section with 100–400 µl of preferred blocking solution for 1 hr at room temperature. Remove blocking solution and add 100–400 µl primary antibody diluted in SignalStain ® Antibody Diluent ( #8112) to each section. Incubate overnight at 4°C.1X Animal-Free Blocking Solution: to 4 mL of dH O add 1 ml of Animal-Free Blocking Solution (5X) ( #15019 ). Incubate sections in three washes of xylene for 5 minutes each. Incubate sections in two washes of 100% ethanol for 10 minutes each. Incubate sections in two washes of 95% ethanol for 10 minutes each.The innate immune system mainly consists of phagocytic cells, such as neutrophils, macrophages, natural killer cells, basophils, and others that target dangerous invading organisms. The cells of the adaptive immune system are T cells and B cells. Natural killer (NK) T cells have features of both innate and adaptive immune cells. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). Protein extracts from 3T3-Src cells were profiled by PhosphoScan ® to identify phosphotyrosine peptides. Tyr458 of PI3K p85 and Tyr199 of PI3K p55 were among 180 phosphopeptides and 185 phosphotyrosine sites identified (5). Cantley, L.C. (2002) Science 296, 1655-7. Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: H-Human M-Mouse R-Rat Hm-Hamster Mk-Monkey Vir-Virus Mi-Mink C-Chicken Dm-D. melanogaster X-Xenopus Z-Zebrafish B-Bovine Dg-Dog Pg-Pig Sc-S. …PAX2 is involved in the development of the nervous and excretory systems including the kidney and urogentical tract, the optic stalk, ear, midbrain-hindbrain junction, and the spinal cord (5,6). Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14. Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62.Lane 1 is 10% input, lane 2 is USP10 (D7A5) Rabbit mAb #8501, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control. Western blot analysis was perfomed using USP10 (D7A5) Rabbit mAb #8501. Anti-rabbit IgG, HRP …Hr -Horse. -Guinea Pig. -Rabbit. -All Species Expected. Monoclonal Antibody for studying NeuN. Cited in 47 publications. Validated for WB, IP, IF. Highly specific and rigorously validated in-house, NeuN (E4M5P) Mouse Monoclonal Antibody (CST …Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.Hr -Horse. -Guinea Pig. -Rabbit. -All Species Expected. Monoclonal Antibody for studying NeuN. Cited in 47 publications. Validated for WB, IP, IF. Highly specific and rigorously validated in-house, NeuN (E4M5P) Mouse Monoclonal Antibody (CST …Background. Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the ...Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.The Harper Lab studies mechanisms underlying cellular homeostasis and signaling, with a focus on the ubiquitin system and the autophagy-lysosome system. The interest in the …In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca 2+ /calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3).Specificity / Sensitivity. Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence.As we continue to create and provide the highest quality of products and services, be part of the team that empowers scientists and clinicians with our best-in-class tools and excellent customer experiences. Engage and share your expertise with our current customers while helping drive results by converting potential clients into profitable ones. Learn & Support. Use this Directory to navigate to a wealth of informative resources about molecular targets and the protocols and tools needed to study them efficiently. Under "Education & Resources" you will find links to a large collection of educational resources, including our PhosphoSitePlus® PTM Database, useful reference tables, guides ... Learn & Support. Use this Directory to navigate to a wealth of informative resources about molecular targets and the protocols and tools needed to study them efficiently. Under "Education & Resources" you will find links to a large collection of educational resources, including our PhosphoSitePlus® PTM Database, useful reference tables, guides ... Flow cytometric analysis of HeLa (blue) and Karpas620 cells (green) using Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F (ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody. Western blot ...p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene ...If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.Cabela's allen texas, Renditions, Moink meat, Hockessin athletic club, Metro care, The palladium worcester, Community integrated services, Montgomery county golf, Brick farm tavern, Kia of amarillo, St marys clothing drive, Diamondhead mississippi, Veterans thrift, Texas real estate commission
Rabbit IgG. Monoclonal Antibody for studying LAMP1. Cited in 477 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, LAMP1 (D2D11) XP® Rabbit Monoclonal Antibody (CST #9091) is ready to ship. . Seea
Western blot analysis of extracts from various cell lines using Alix (E6P9B) Rabbit mAb. Immunoprecipitation of Alix from K-562 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control, and lane 3 is Alix (E6P9B) Rabbit mAb. Western blot was performed using Alix (E6P9B) Rabbit mAb.Apr 15, 2022 · Cell Signaling Technology (CST) emphasizes that it was founded by scientists, for scientists. This is at the core of the company’s culture and permeates all its decisions. Headed by neuroscientist Michael Comb, CST seeks to deeply understand the requirements of their customers—bench researchers who are in the weeds of complex research ... Lane 1 is 10% input, lane 2 is USP18 (D4E7) Rabbit mAb, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900. Western blot analysis was perfomed using USP18 (D4E7) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. To Purchase # 4813. Cat. Aliquoting of 10x buffer is recommended if many small experiments are to be performed. 2. Thaw 10x buffer at 24-30°C, mixing end-over-end. 3. Dilute 10X Cell Lysis Buffer to a 1X solution using ddH2O. This product supplies enough 10X material to make 150mls of whole cell extract. 4. Chill 1X buffer on ice and add PMSF just prior to use. Simple Western™ analysis of lysates (0.1 mg/mL) from Jurkat cells treated with Cytochrome C using Cleaved Caspase-3 (Asp175) Antibody #9661. The virtual lane view (left) shows two target bands (as indicated) at 1:10 and 1:50 dilutions of primary antibody. Prepare 1X SignalFire™ ECL Reagent ( #6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film. Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min.If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.CUT&RUN was performed with 100,000, 10,000, or 5,000 HCT 116 cells (as indicated) and Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751, using the CUT&RUN Assay Kit. DNA Libraries were prepared using DNA Library Prep Kit for Illumina ® (ChIP-seq, CUT&RUN) #56795. This figure shows enrichment around the GAPDH gene, a known …1X Animal-Free Blocking Solution: to 4 mL of dH O add 1 ml of Animal-Free Blocking Solution (5X) ( #15019 ). Incubate sections in three washes of xylene for 5 minutes each. Incubate sections in two washes of 100% ethanol for 10 minutes each. Incubate sections in two washes of 95% ethanol for 10 minutes each.NOTE: Most antibodies from Cell Signaling Technology work optimally between 1 and 2 ug per IP sample. In the case where there are multiple samples with varying concentrations, …DIVERSITY, EQUITY, & INCLUSION. CST understands the importance of a safe and diverse workplace. Different perspectives and ideas drive collaboration and innovation. By providing an inclusive and stimulating work environment, we aim to establish human dignity and meaningful work as an essential part of our culture. Embrace the. culture.Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows ...Lane 1 is 10% input, lane 2 is USP10 (D7A5) Rabbit mAb #8501, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control. Western blot analysis was perfomed using USP10 (D7A5) Rabbit mAb #8501. Anti-rabbit IgG, HRP …Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). Heat a 20 µl sample to 95–100°C for 5 min; cool on ice. Microcentrifuge for 5 min. Rabbit IgG. Monoclonal Antibody for studying LAMP1. Cited in 477 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, LAMP1 (D2D11) XP® Rabbit Monoclonal Antibody (CST #9091) is ready to ship. Place the tubes on the magnetic rack until the solution turns clear (30 sec to 2 min) and then remove the liquid. Remove tubes from magnetic rack. Add 50 µl of pAG-MNase pre-mix to each tube and gently mix the sample by pipetting up and down. Incubate tubes at 4°C for 1 …Place the tubes on the magnetic rack until the solution turns clear (30 sec to 2 min) and then remove the liquid. Remove tubes from magnetic rack. Add 50 µl of pAG-MNase pre-mix to each tube and gently mix the sample by pipetting up and down. Incubate tubes at 4°C for 1 …The Rb (4H1) Mouse mAb confirms silencing of Rb expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Rb siRNA. Western blot analysis of extracts from COS-7 cells, untreated or hydroxyurea-treated (G1/S), using Rb (4H1) Mouse mAb. Immunohistochemical analysis of paraffin-embedded human breast ... Learn & Support. Use this Directory to navigate to a wealth of informative resources about molecular targets and the protocols and tools needed to study them efficiently. Under "Education & Resources" you will find links to a large collection of educational resources, including our PhosphoSitePlus® PTM Database, useful reference tables, guides ... Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. Cathepsin B (D1C7Y) XP ® Rabbit mAb recognizes endogenous levels of total cathepsin B protein. Lane 1 is 10% input, lane 2 is USP10 (D7A5) Rabbit mAb #8501, and lane 3 is Rabbit (DA1E) mAb IgG XP ® Isotype Control. Western blot analysis was perfomed using USP10 (D7A5) Rabbit mAb #8501. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. Show More. If you're looking for a career that you can feel good about with a company that cares about you, the environment, and our communities, look no further than Cell Signaling Technology. Check out our current career … Applications. Scientists at Cell Signaling Technology (CST) use a combination of several approaches to validate antibodies in a number of applications, to provide you with the highest quality and most thoroughly tested reagents. The majority of our products are initially tested and approved by western blotting. If western analysis shows a ... CUT&RUN was performed with HeLa cells and Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina ® (ChIP …Partnerships & Licensing. As a trusted partner, CST is focused on building symbiotic relationships to maximize the value of products, technology, and intellectual property; whether ours or our partner’s. True innovation is collaborative. We have broad interest in technology licensing, scientific and technical collaborations, and commercial ... ELISA: An Introduction. Enzyme-linked immunosorbent assay (ELISA) allows for the quantification of a molecule of interest using antibodies and tags (such as the enzyme horseradish peroxidase [HRP]), either directly or indirectly coupled to the antibody for detection. ELISA can be used with a variety of sample types, such as cell lysate, tissue ... Application Key: WB-Western IP-Immunoprecipitation IHC-Immunohistochemistry ChIP-Chromatin Immunoprecipitation IF-Immunofluorescence F-Flow Cytometry Species Cross-Reactivity Key: H-Human M-Mouse R-Rat Hm-Hamster Mk-Monkey Vir-Virus Mi-Mink C-Chicken Dm-D. melanogaster X-Xenopus Z-Zebrafish B-Bovine Dg-Dog Pg-Pig Sc-S. cerevisiae Ce-C. …Checking the Cell Signaling Technology ® (CST ®) website, you are in luck! There are mouse reactive flow-validated antibody conjugates available against each target: CD4 …Background. Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the ...Product Description. This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis in human cells. This antibody conjugate is expected to exhibit the same reactivity as the unconjugated G4S Linker (E7O2V) Rabbit mAb #71645, which is ...The interactive pathways and diagrams have been created by CST scientists and outside experts to provide concise overviews of selected signaling pathways and cellular mechanisms. Protein nodes in each interactive diagram are linked to specific antibody product information, as well as protein-specific listings in the PhosphoSitePlus ® …Specificity / Sensitivity. Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence.Apr 15, 2022 · Cell Signaling Technology (CST) emphasizes that it was founded by scientists, for scientists. This is at the core of the company’s culture and permeates all its decisions. Headed by neuroscientist Michael Comb, CST seeks to deeply understand the requirements of their customers—bench researchers who are in the weeds of complex research ... Cell Signaling Technology poster included with each book. The two-sided poster details important signaling pathways. Chapter 12, How Cells Make Decisions, offers a unique visual and interrogative approach that …Western blot analysis of extracts from various cell lines using GCN5L2 (C26A10) Rabbit mAb. Confocal immunofluorescent analysis of HeLa cells using GCN5L2 (C26A10) Rabbit mAb (green). Actin filaments have been labeled with DY554 phalloidin (red). Blue pseudocolor = DRAQ5 ™ (fluorescent DNA dye). To Purchase # 3305. Cat.Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63411.Polyclonal Antibody for studying H3 (Ser10) phosphate. Cited in 1110 publications. Validated for WB, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Histone H3 (Ser10) Antibody (CST #9701) is ready to ship.Immunoprecipitation of TIM-3 from RPMI 8226 cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is TIM-3 (D5D5R ™) XP ® Rabbit mAb, #45208. Western blot was performed using TIM-3 (D5D5R ™) XP ® Rabbit mAb.Learn & Support. Use this Directory to navigate to a wealth of informative resources about molecular targets and the protocols and tools needed to study them efficiently. Under …Monoclonal Antibody for studying Flag-Tag. Cited in 478 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, DYKDDDDK Tag (9A3) Mouse Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (CST #8146) is ready to ship.The LC3B (D11) XP ® Rabbit mAb confirms silencing of LC3B expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of LC3B siRNA. Show More. Simple Western™ analysis of lysates (1mg/ml) from HeLa cells treated with Chloroquine (50uM, O/N) using LC3B (D11) XP ® Rabbit mAb #3868. Learn & Support. Use this Directory to navigate to a wealth of informative resources about molecular targets and the protocols and tools needed to study them efficiently. Under "Education & Resources" you will find links to a large collection of educational resources, including our PhosphoSitePlus® PTM Database, useful reference tables, guides ... Science & Development. As a company of scientists for scientists, we are passionate about the discovery and development of innovative therapies for human disease. Help advance …RSS Feed. Cell signalling is the mechanism by which stimuli are transmitted via a signalling cascade to effector molecules that orchestrate the appropriate response. Types of cell signalling ...Polyclonal Antibody for studying H3 (Ser10) phosphate. Cited in 1110 publications. Validated for WB, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Histone H3 (Ser10) Antibody (CST #9701) is ready to ship.CiteAb’s 2022 analysis shows Cell Signaling Technology has maintained a leading position as the vendor with the most antibodies in the top 100. This figure now stands at 36, an …Place the tubes on the magnetic rack until the solution turns clear (30 sec to 2 min) and then remove the liquid. Remove tubes from magnetic rack. Add 50 µl of pAG-MNase pre-mix to each tube and gently mix the sample by pipetting up and down. Incubate tubes at 4°C for 1 …Flow cytometric analysis of HeLa (blue) and Karpas620 cells (green) using Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F (ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody. Western blot ...Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. Cathepsin B (D1C7Y) XP ® Rabbit mAb recognizes endogenous levels of total cathepsin B protein. 抗体不应成为您实验中的变量之一。了解客户为何在抗体特异性和敏感性方面给予 cst 最高评价。 Monoclonal Antibody for studying ACTA2 (alpha actin, smooth muscle). Cited in 560 publications. Validated for WB, WB, IP, IHC, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, α-Smooth Muscle Actin (D4K9N) XP® Rabbit Monoclonal Antibody (CST #19245) is ready to ship.Western blot analysis of extracts from various cell lines using Alix (E6P9B) Rabbit mAb. Immunoprecipitation of Alix from K-562 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control, and lane 3 is Alix (E6P9B) Rabbit mAb. Western blot was performed using Alix (E6P9B) Rabbit mAb.If you're looking for a career that you can feel good about with a company that cares about you, the environment, and our communities, look no further than Cell Signaling Technology. Check out our current career … For reproducible data, Cell Signaling Technology (CST) offers rigorously validated and reliable products including antibodies, reagents, and kits for biological research. We have a substantial portfolio of products to interrogate mechanisms of cellular health and disease. Whether in the fields of cancer, immunology, infectious disease ... Product Description. This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis in human cells. This antibody conjugate is expected to exhibit the same reactivity as the unconjugated G4S Linker (E7O2V) Rabbit mAb #71645, which is ...Product Description. This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis in human cells. This antibody conjugate is expected to exhibit the same reactivity as the unconjugated G4S Linker (E7O2V) Rabbit mAb #71645, which is ...Western blot analysis of extracts from various cell lines using GAPDH (14C10) Rabbit mAb. Simple Western™ analysis of lysates (0.01 mg/mL) from HeLa cells using GAPDH (14C10) Rabbit mAb #2118. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody.Cell Signaling Technology (CST) is a different kind of life sciences company—one founded, owned, and run by active research scientists, with the highest standards of product and service quality ...NKX2.5 plays an essential role in heart development and is among the earliest factors expressed in the cardiac lineage in developing embryos. Targeted disruption of the murine Nkx2.5 gene results in abnormal heart morphogenesis, severe growth retardation, and embryonic lethality around E9.5 (1,2).DIVERSITY, EQUITY, & INCLUSION. CST understands the importance of a safe and diverse workplace. Different perspectives and ideas drive collaboration and innovation. By providing an inclusive and stimulating work environment, we aim to establish human dignity and meaningful work as an essential part of our culture. Embrace the. culture.Learn & Support. Use this Directory to navigate to a wealth of informative resources about molecular targets and the protocols and tools needed to study them efficiently. Under …Immunoprecipitation of TIM-3 from RPMI 8226 cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is TIM-3 (D5D5R ™) XP ® Rabbit mAb, #45208. Western blot was performed using TIM-3 (D5D5R ™) XP ® Rabbit mAb.Ferroptosis is an iron-dependent form of regulated cell death associated with an increase in lipid peroxides (reviewed in 1,2). Free divalent iron (Fe2+) can lead to spontaneous lipid peroxidation through a Fenton reaction. Ferroptosis is regulated by signaling pathways that control iron storage and oxidative stress.The western blot technique is a powerful tool to elucidate the complex signaling events that underlie biological processes and disease. This paper highlights critical steps in the …If using antibodies from Cell Signaling Technology, please see recommended dilution listed on the datasheet or product webpage and calculate the amount (µg) of IgG antibody for negative control based on the Cell Signaling Antibody concentration for a fair comparison. Incubate IP samples 4 h to overnight at 4°C with rotation.The Rb (4H1) Mouse mAb confirms silencing of Rb expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Rb siRNA. Western blot analysis of extracts from COS-7 cells, untreated or hydroxyurea-treated (G1/S), using Rb (4H1) Mouse mAb. Immunohistochemical analysis of paraffin-embedded human breast ... CiteAb’s 2022 analysis shows Cell Signaling Technology has maintained a leading position as the vendor with the most antibodies in the top 100. This figure now stands at 36, an increase of one over 2021, which is double that of the second most cited supplier. In addition, our antibodies now hold all three top spots, with our Anti-rabbit IgG ... Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (50 μM, 1 μM, and 10 μM, 2 hr; blue) using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (dashed lines). Anti-rabbit IgG …Use the Cell Signaling Technology Technical Documentation search tool to find Product Data Sheets, Certificates of Analysis (CoA), and Safety Data Sheets (SDS). Begin your search by entering the product number in the search field. Online publication of this documentation makes it easier for you to access it, and more environmentally friendly .... Streamsong resort florida, Arrangeme, Fragnancenet, The sub shop, Four seasons santa fe, Colorado newsline, Cliff at lic, Rental pro, Klick lewis.